HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SLU7 (Cat# RC202113, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLU7 (Cat# TA810915)(1:500). Positive lysates [LY416650] (100 ug) and [LC416650] (20 ug) can be purchased separately from OriGene.