HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NR1H4 (Cat# RC217443, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1H4 (Cat# TA809306)(1:2000). Positive lysates [LY401577] (100 ug) and [LC401577] (20 ug) can be purchased separately from OriGene.