HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SHMT2 (Cat# RC204239, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHMT2 (Cat# TA808820)(1:2000). Positive lysates [LY401661] (100 ug) and [LC401661] (20 ug) can be purchased separately from OriGene.