HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR2F6 (RC201336, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR2F6. Positive lysates LY401603 (100 ug) and LC401603 (20 ug) can be purchased separately from OriGene.