HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SERBP1 (Cat# RC204527, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERBP1(Cat# TA800707). Positive lysates [LY422719] (100 ug) and [LC422719] (20 ug) can be purchased separately from OriGene.