HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SF3A1 (RC201098, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SF3A1. Positive lysates LY416998 (100 ug) and LC416998 (20 ug) can be purchased separately from OriGene.