HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD274 (Cat# RC213071, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274(Cat# TA507087). Positive lysates [LY415473] (100 ug) and [LC415473] (20 ug) can be purchased separately from OriGene.