HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY EIF5A2 (Cat# RC206249, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF5A2(Cat# TA505100). Positive lysates [LY412495] (100 ug) and [LC412495] (20 ug) can be purchased separately from OriGene.