HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PPP1R15A (Cat# RC200581, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP1R15A(Cat# TA504310). Positive lysates [LY415358] (100 ug) and [LC415358] (20 ug) can be purchased separately from OriGene.