HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PDIA4 (Cat# RC204041, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDIA4(Cat# TA503882). Positive lysates [LY401531] (100 ug) and [LC401531] (20 ug) can be purchased separately from OriGene.