HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SMAD2 (Cat# RC204604, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMAD2(Cat# TA501728). Positive lysates [LY401783] (100 ug) and [LC401783] (20 ug) can be purchased separately from OriGene.