HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIL1 (RC211850, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIL1. Positive lysates LY402924 (100 ug) and LC402924 (20 ug) can be purchased separately from OriGene.