HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSD17B10 (RC201734, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSD17B10 (TA500724). Positive lysates LY401426 (100 ug) and LC401426 (20 ug) can be purchased separately from OriGene.