HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP5C (RC201650, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP5C. Positive lysates LY416767 (100 ug) and LC416767 (20 ug) can be purchased separately from OriGene.