HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BRAF (Cat# RC211013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF(Cat# TA500431). Positive lysates [LY401382] (100 ug) and [LC401382] (20 ug) can be purchased separately from OriGene.