Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and CHEK2-Knockout hela cells (KO, Cat# LC810324) were separated by SDS-PAGE and immunoblotted with anti-CHEK2 monoclonal antibody TA500398. Then the blotted membrane was stripped and reprobed with anti-β-actin (TA811000) as a loading control (1:500).