HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY KRT18 (Cat# RC210049, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT18(Cat# TA500015). Positive lysates [LY404683] (100 ug) and [LC404683] (20 ug) can be purchased separately from OriGene.