HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZsYellow1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZsYellow1 (TA180004, 1:2000).