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Cat Number:CCH385-B100ML
Size:each
Concentration:Sufficient for over 25 mini-gel size membranes.
Price:£250.00
Quantity:
Shipping:£12.00

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Data Sheet

 

UltraScence Atto Western Substrate [100ml [50 ml x 2]]

Partner: Bio-Helix Co., Ltd

Description:The UltraScence Atto Western Substrate, as a luminol-based enhanced chemiluminescent substrate, is the most sensitive and brightest ECL Western Substrate among our UltraScence product lines for low-femtogram to high-attogram detection of antigen with excellent sensitivity and long signal duration. UltraScence Atto Western Substrate is compatible with conducting immunoblots with horseradish peroxidase (HRP) � conjugated secondary antibodies. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal.
Further Information:US Patent No.: US10,711,185
Shipping Conditions:Ship at ambient
Storage:Store at 4 C for 2 years
Usage:Research use only

 

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Membranes were probed with GFP tag Rabbit PolyAb diluted at 1:10,000 of and then with Goat Anti-rabbit IgG/HRP secondary antibody (1:10,000) after serial dilution EGFP (Enhanced Green Fluorescent Protein) were prepared and applied in electrophoresis and protein transfer. Identical blots were incubated with the Western substrate. The blots were simultaneously exposed for 5 seconds, 10 seconds, 30 seconds, 60 seconds, and 180 seconds using ChemluxSPX-600 Series digital imaging system.

Membranes were probed with GFP tag Rabbit PolyAb diluted at 1:10,000 of and then with Goat Anti-rabbit IgG/HRP secondary antibody (1:10,000) after serial dilution EGFP (Enhanced Green Fluorescent Protein) were prepared and applied in electrophoresis and protein transfer. Identical blots were incubated with the Western substrate. The blots were simultaneously exposed for 5 seconds, 10 seconds, 30 seconds, 60 seconds, and 180 seconds using ChemluxSPX-600 Series digital imaging system.