All lanes : Anti-(DANRE) gnb1 Antibody (center) at 1:1000 dilutionLane 1: Zebrafish lysateLane 2: ZF4 whole cell lysate Lysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDaBlocking/Dilution buffer: 5% NFDM/TBST.