All lanes : Anti-(DANRE) heg Antibody (C-term) at 1:1000 dilutionLane 1: Zebrafish muscle lysateLane 2: Zebrafish lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 106 kDaBlocking/Dilution buffer: 5% NFDM/TBST.