Ordering Details
Cat Number:	ATR0917-100ul
Size:	100uL
Concentration:	1mg/mL
Price:	£205.00
Quantity:
Shipping:	£12.00

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MSDS

TREM-2 [2B5], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, ICC/IF, FACS
Species Reactivity:	Human
Isotype:	Mouse IgG3 kappa
Immunogen:	Recombinant human TREM2 (19-161aa) purified from E. coli
Purification:	By protein-G affinity chromatography
Conjugation:	Unconjugated
Description:	The triggering receptor expressed on myeloid cells-2 (TREM2) is a membrane protein that interacts with the TYROBP protein. The TREM2 and TYROBP proteins form a complex that transmits chemical signals to activate the cell. This complex is involved in the growth and development of several types of immune cells, particularly dendritic cells and activates these cells, triggering an inflammatory response to injury or disease. And the TYROBP-TREM2 complex also activates cells in the skeletal system and in the brain and spinal cord (central nervous system).
Further Information:	The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.
Synonym:	triggering receptor expressed on myeloid cells 2 isoform 1, triggering receptor expressed on myeloid cells 2, triggering receptor expressed on myeloid cells 2a, TREM-2, Trem2a, Trem2b, Trem2c
Ascension Number:	Q9NZC2
NCBI EntrezGene:	NP_061838
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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Cell lysates of HeLa, MCF7, LNCaP and mouse brain (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TREM2 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ICC/IF analysis of TREM2 in HepG2 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TREM2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).ICC/IF analysis of TREM2 in Raw264.7 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TREM2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).