Ordering Details
Cat Number:	ATR0601-50ul
Size:	50uL
Concentration:	1mg/mL
Price:	£123.00
Quantity:
Shipping:	£12.00

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MSDS

TLR7 [4F4], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, FACS, ICC/IF
Species Reactivity:	Human
Isotype:	Mouse IgG1 kappa
Immunogen:	Recombinant human TLR7 (451-500aa) purified from E. coli
Purification:	By protein-G affinity chromatography
Conjugation:	Unconjugated
Description:	TLR7 is one of toll-like receptors which are type I transmembrane proteins involved in innate immunity by recognizing microbial conserved structures. These proteins are characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain, and act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. Human TLR7 (hTLR7) is predominantly expressed in lung, placenta and spleen and hTLR7, 8 genes are located on the sex chromosome X. hTLR7 may play an important role in detecting and combating viral infections.
Further Information:	The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000.
Synonym:	toll-like receptor 7, PRO285, UNQ248, tlr 7
Ascension Number:	Q9NYK1
NCBI EntrezGene:	NP_057646
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The Ramos cell lysates (80ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TLR7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Arrow indicates TLR7 protein in human Ramos cells.

ICC/IF analysis of TLR7 in THP-1 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TLR7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).