| Applications: | SDS-PAGE |
| Species Reactivity: | Human |
| MW: | 62.3kDa (549aa) |
| Purity: | > 90% by SDS - PAGE |
| Description: | B3GAT1, also known as galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, is a key enzyme during the biosynthesis of the carbohydrate epitope HNK-1. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This protein is expressed as a carbohydrate epitope that contains a sulfoglucuronyl residue in several adhesion molecules of the nervous system. Also, the enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol. This protein functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1. Recombinant Human B3GAT1, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Further Information: | Sequence: <ADP>TLAPLLA VHKDEGSDPR RETPPGADPR EYCTSDRDIV EVVRTEYVYT RPPPWSDTLP TIHVVTPTYS RPVQKAELTR MANTLLHVPN LHWLVVEDAP RRTPLTARLL RDTGLNYTHL HVETPRNYKL RGDARDPRIP RGTMQRNLAL RWLRETFPRN SSQPGVVYFA DDDNTYSLEL FEEMRSTRRV SVWPVAFVGG LRYEAPRVNG AGKVVGWKTV FDPHRPFAID MAGFAVNLRL ILQRSQAYFK LRGVKGGYQE SSLLRELVTL NDLEPKAANC TKILVWHTRT EKPVLVNEGK KGFTDPSVEI <LEPKSCDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV> <DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS> <NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN> <GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN> <HYTQKSLSLS PGKHHHHHH> |
| Synonym: | B3GAT1, CD57, GLCATP, GLCUATP, HNK1, LEU7, NK-1, NK1, Beta-1,3-glucuronyltransferase 1, Glucuronosyltransferase P, GlcAT-P, UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase, GlcUAT-P |
| Ascension Number: | Q9P2W7 |
| NCBI EntrezGene: | NP_473366 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
