| Applications: | SDS-PAGE |
| Species Reactivity: | Human |
| MW: | 75.6kDa (678aa);;70-100kDa (SDS-PAGE under reducing conditions) |
| Purity: | > 85% by SDS - PAGE |
| Description: | Siglec-10, also known as Sialic acid binding Ig like lectin 10 isoform 3, is a member of the immunoglobulin superfamily that is expressed on eosinophils, neutrophils, monocytes, and B cells. This protein bears an Immuno-receptor tyrosine based inhibitory motif within its cytoplasmic domain. It is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. Also, it binds to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24. This binding can be modulated by cis interactions of Siglec-10 with sialated molecules on the same cell. Recombinant human Siglec-10, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Further Information: | Sequence: MDGRFWIRVQ ESVMVPEGLC ISVPCSFSYP RQDWTGSTPA YGYWFKAVTE TTKGAPVATN HQSREVEMST RGRFQLTGDP AKGNCSLVIR DAQMQDESQY FFRVERGSYV RYNFMNDGFF LKVTALTQKP DVYIPETLEP GQPVTVICVF NWAFEECPPP SFSWTGAALS SQGTKPTTSH FSVLSFTPRP QDHNTDLTCH VDFSRKGVSA QRTVRLRVAY APRDLVISIS RDNTPALEPQ PQGNVPYLEA QKGQFLRLLC AADSQPPATL SWVLQNRVLS SSHPWGPRPL GLELPGVKAG DSGRYTCRAE NRLGSQQRAL DLSVQYPPEN LRVMVSQANR TVLENLGNGT SLPVLEGQSL CLVCVTHSSP PARLSWTQRG QVLSPSQPSD PGVLELPRVQ VEHEGEFTCH ARHPLGSQHV SLSLSVHYKK GLISTAFSN<L EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR> <TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN> <GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS> <DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH> <YTQKSLSLSP GKHHHHHH> |
| Synonym: | SIGLEC10, PRO940, SLG2, SIGLEC-10, Sialic acid-binding Ig-like lectin 10 isoform 3, Siglec-like protein 2 |
| Ascension Number: | Q96LC7 |
| NCBI EntrezGene: | NP_001164628 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
