| Applications: | SDS-PAGE |
| Species Reactivity: | Human |
| MW: | 37.7kDa (338aa);;40-57kDa (SDS-PAGE under reducing conditions) |
| Purity: | > 90% by SDS - PAGE |
| Description: | HP, also known as haptoglobin isoform 2, is consists of two alpha and two beta chains, connected by disulfide bridges in its simplest form. The chains originate from a common precursor protein, which is proteolytically cleaved during protein synthesis. In blood plasma, this protein binds free hemoglobin released from erythrocytes with high affinity and thereby inhibits its oxidative activity. The haptoglobin-hemoglobin complex will then be removed by the reticuloendothelial system (mostly the spleen). It functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. For this reason, it is often referred to as the suicide protein. Recombinant human HP, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Further Information: | Sequence: ADLVDSGNDV TDIADDGCPK PPEIAHGYVE HSVRYQCKNY YKLRTEGDGV YTLNNEKQWI NKAVGDKLPE CEAVCGKPKN PANPVQRILG GHLDAKGSFP WQAKMVSHHN LTTGATLINE QWLLTTAKNL FLNHSENATA KDIAPTLTLY VGKKQLVEIE KVVLHPNYSQ VDIGLIKLKQ KVSVNERVMP ICLPSKDYAE VGRVGYVSGW GRNANFKFTD HLKYVMLPVA DQDQCIRHYE GSTVPEKKTP KSPVGVQPIL NEHTFCAGMS KYQEDTCYGD AGSAFAVHDL EEDTWYATGI LSFDKSCAVA EYGVYVKVTS IQDWVQKTIA ENHHHHHH |
| Synonym: | Haptoglobin isoform 2, HP, BP, HP2ALPHA2, HPA1S |
| Ascension Number: | P00738 |
| NCBI EntrezGene: | NP_001119574 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
