| Applications: | Enzyme Activity, SDS-PAGE |
| Species Reactivity: | Human |
| MW: | 15.9 kDa (145aa), confirmed by MALDI-TOF |
| Purity: | > 95% by SDS - PAGE |
| Description: | PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
| Further Information: | Sequence: MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY |
| Synonym: | 14 kDa phosphohistidine phosphatas, bA216L13.10, CGI-202, DKFZp564M173, HSPC141, PHP14, RP11-216L13.10 |
| Ascension Number: | Q9NRX4 |
| NCBI EntrezGene: | NP_054891 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
