| Applications: | SDS-PAGE |
| Species Reactivity: | Human |
| MW: | 36.2 kDa (310aa) confirmed by MALDI-TOF |
| Purity: | > 90% by SDS - PAGE |
| Description: | Heparan sulfate glucosamine 3-O-sulfotransferase 1, also known as HS3ST1, is sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. This protein catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site. Recombinant human HS3ST1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
| Further Information: | Sequence: MGSSHHHHHH SSGLVPRGSH MGSRPAELGQ QELLRKAGTL QDDVRDGVAP NGSAQQLPQT IIIGVRKGGT RALLEMLSLH PDVAAAENEV HFFDWEEHYS HGLGWYLSQM PFSWPHQLTV EKTPAYFTSP KVPERVYSMN PSIRLLLILR DPSERVLSDY TQVFYNHMQK HKPYPSIEEF LVRDGRLNVD YKALNRSLYH VHMQNWLRFF PLRHIHIVDG DRLIRDPFPE IQKVERFLKL SPQINASNFY FNKTKGFYCL RDSGRDRCLH ESKGRAHPQV DPKLLNKLHE YFHEPNKKFF ELVGRTFDWH |
| Synonym: | Heparan sulfate glucosamine 3-O-sulfotransferase 1, Heparan sulfate glucosamine 3-O-sulfotransferase 1, 3OST, 3OST1 |
| Ascension Number: | O14792 |
| NCBI EntrezGene: | NP_005105 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
