| Applications: | SDS-PAGE, Denatured |
| Species Reactivity: | Human |
| MW: | 32.2 kDa (285aa) |
| Purity: | > 85% by SDS - PAGE |
| Description: | POu domain, class 5, transcription factor 1, also known as OCT4, binds to the octamer motif (5'-ATTTGCAT-3'). This protein forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, uTF1 and ZFP206. It is critical for early embryogenesis and for embryonic stem cell pluripotency. Recombinant human OCT4 protein, fused to His-tag at N-terminus, was expressed in E. coli. |
| Further Information: | Sequence: MGSSHHHHHH SSGLVPRGSH MHFYRLFLGA TRRFLNPEWK GEIDNWCVYV LTSLLPFKIQ SQDIKALQKE LEQFAKLLKQ KRITLGYTQA DVGLTLGVLF GKVFSQTTIC RFEALQLSFK NMCKLRPLLQ KWVEEADNNE NLQEICKAET LVQARKRKRT SIENRVRGNL ENLFLQCPKP TLQQISHIAQ QLGLEKDVVR VWFCNRRQKG KRSSSDYAQR EDFEAAGSPF SGGPVSFPLA PGPHFGTPGY GSPHFTALYS SVPFPEGEAF PPVSVTTLGS PMHSN |
| Synonym: | POU domain, class 5, transcription factor 1 isoform 3, Oct-3, Oct-4, OCT3, OCT4, OTF-3, OTF3, OTF4 |
| Ascension Number: | Q01860 |
| NCBI EntrezGene: | NP_001272916 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
