| Applications: | SDS-PAGE |
| Species Reactivity: | E.coli |
| MW: | 20 kDa (178aa), confirmed by MALDI-TOF |
| Purity: | > 95 % by SDS - PAGE |
| Description: | rnhA is an endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Localized to the nucleus, this protein mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. rnhA catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is able to bind magnesium or manganese as cofactors. Recombinant e. coli rnhA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
| Further Information: | Sequence: MGSSHHHHHH SSGLVPRGSH MGSMLKQVEI FTDGSCLGNP GPGGYGAILR YRGREKTFSA GYTRTTNNRM ELMAAIVALE ALKEHCEVIL STDSQYVRQG ITQWIHNWKK RGWKTADKKP VKNVDLWQRL DAALGQHQIK WEWVKGHAGH PENERCDELA RAAAMNPTLE DTGYQVEV |
| Synonym: | Ribonuclease HI degrades RNA of DNA-RNA hybrids., Ribonuclease HI, degrades RNA of DNA-RNA hybrids., cer, dasF, ECK0214, herA, JW0204, rnh, sdrA, sin |
| Ascension Number: | P0A7Y4 |
| NCBI EntrezGene: | NP_414750 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
