| Applications: | Enzyme Activity, SDS-PAGE |
| Species Reactivity: | E.coli |
| MW: | 29.3kDa (273aa) confirmed by MALDI-TOF |
| Purity: | > 95% by SDS - PAGE |
| Description: | uridine phosphorypase (uDP) catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. This enzyme belongs to the family of glycosyltransferases, specifically the pentosyltransferases. The systematic name of this enzyme class is uridine:phosphate alpha-D-ribosyltransferase. Other names in common use include pyrimidine phosphorylase, urdPase, uPH, and uPase. Recombinant E. coli uDP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
| Further Information: | Sequence: MGSSHHHHHH SSGLVPRGSH MSKSDVFHLG LTKNDLQGAT LAIVPGDPDR VEKIAALMDK PVKLASHREF TTWRAELDGK PVIVCSTGIG GPSTSIAVEE LAQLGIRTFL RIGTTGAIQP HINVGDVLVT TASVRLDGAS LHFAPLEFPA VADFECTTAL VEAAKSIGAT THVGVTASSD TFYPGQERYD TYSGRVVRHF KGSMEEWQAM GVMNYEMESA TLLTMCASQG LRAGMVAGVI VNRTQQEIPN AETMKQTESH AVKIVVEAAR RLL |
| Synonym: | uridine phosphorylase, urdPase, upase, uridine phosphorylase |
| Ascension Number: | P12758 |
| NCBI EntrezGene: | NP_418275 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
