| Applications: | SDS-PAGE |
| Species Reactivity: | E.coli |
| MW: | 26.0 kDa (237aa) confirmed by MALDI-TOF |
| Purity: | > 95% by SDS - PAGE |
| Description: | NFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5- (aziridin-1-yl) -2, 4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species. Recombinant E. coli NFNB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
| Further Information: | Sequence: MGSSHHHHHH SSGLVPRGSH MDIISVALKR HSTKAFDASK KLTPEQAEQI KTLLQYSPSS TNSQPWHFIV ASTEEGKARV AKSAAGNYVF NERKMLDASH VVVFCAKTAM DDVWLKLVVD QEDADGRFAT PEAKAANDKG RKFFADMHRK DLHDDAEWMA KQVYLNVGNF LLGVAALGLD AVPIEGFDAA ILDAEFGLKE KGYTSLVVVP VGHHSVEDFN ATLPKSRLPQ NITLTEV |
| Synonym: | dihydropteridine reductase NAD(P)H-dependent oxygen-insensitive, dprA, nfsB, nfsI, ntr, dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive |
| Ascension Number: | P38489 |
| NCBI EntrezGene: | NP_415110 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
