| Applications: | SDS-PAGE |
| Species Reactivity: | Human |
| MW: | 21.4 kDa (188aa), confirmed by MALDI-TOF |
| Purity: | > 90% by SDS - PAGE |
| Description: | uBE2B, also known as ubiquitin-conjugating enzyme E2 B, belongs to E2 ubiquitin-conjugating enzyme family. This protein catalyzes the covalent attachment of ubiquitin to other proteins, and associates to the E3 ligase RAD18 to form the uBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. It is required for postreplication repair of uV-damaged DNA. It may be involved in neurite outgrowth. Recombinant human uBE2B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
| Further Information: | Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSTP ARRRLMRDFK RLQEDPPVGV SGAPSENNIM QWNAVIFGPE GTPFEDGTFK LVIEFSEEYP NKPPTVRFLS KMFHPNVYAD GSICLDILQN RWSPTYDVSS ILTSIQSLLD EPNPNSPANS QAAQLYQENK REYEKRVSAI VEQSWNDS |
| Synonym: | ubiquitin-conjugating enzyme E2B, E2-17kDa, HHR6B, HR6B, RAD6B, uBC2, ubiquitin-conjugating enzyme E2B hHR6B, RAD6 homolog B, ubiquitin carrier protein B, ubiquitin conjugating enzyme E2 B, ubiquitin protein ligase B, ubiquitin-conjugating enzyme E2 17 kDa. |
| Ascension Number: | P63146 |
| NCBI EntrezGene: | NP_003328 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
