Ordering Details
Cat Number:	ATGP0537-500ug
Size:	500ug
Concentration:	1 mg/mL (determined by Bradford assay)
Price:	£520.00
Quantity:
Shipping:	£12.00

Sonic Hedgehog/Shh [25-198 a.a.], Recombinant Protein

Applications:	SDS-PAGE
Species Reactivity:	Mouse
MW:	20.8 kDa (183aa), confirmed by MALDI-TOF
Purity:	> 95% by SDS-PAGE
Description:	SHH is one of three proteins in the mammalian signaling pathway family called hedgehog, the others being desert hedgehog (DHH) and Indian hedgehog (IHH). This protien is the best studied ligand of the hedgehog signaling pathway. It plays a key role in regulating vertebrate organogenesis. SHH contain amino-terminal signal peptides and apparently function as secreted proteins involved in the mediation of various cell-cell interactions. Recombinant mouse SHH protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Further Information:	Sequence: MCGPGRGFGK RRHPKKLTPL AYKQFIPNVA EKTLGASGRY EGKITRNSER FKELTPNYNP DIIFKDEENT GADRLMTQRC KDKLNALAIS VMNQWPGVKL RVTEGWDEDG HHSEESLHYE GRAVDITTSD RDRSKYGMLA RLAVEAGFDW VYYESKAHIH CSVKAENSVA AKSGGLEHHH HHH
Synonym:	Sonic hedgehog protein, Dsh, Hhg1, Hx, Hx13, Sonic hedgehog protein HHG 1, HHG1, HLP 3, HLP3, Holoprosencephaly 3, HPE 3, HPE3, MCOPCB5, SHH, SMMC I, SMMCI, Sonic Hedgehog (Drosophila) homolog, sonic hedgehog homolog (Drosophila), Sonic hedgehog homolog, TPT, TPTPS.
Ascension Number:	Q62226
NCBI EntrezGene:	NP_033196
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only