| Applications: | SDS-PAGE |
| Species Reactivity: | Human |
| MW: | 24.5 kDa (217aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher) |
| Purity: | > 90% by SDS - PAGE |
| Description: | ASPH (Aspartate beta-hydroxylase) is a widely-expressed type II membrane protein involved in calcium homeostasis. Located in the endoplasmic reticulum, ASPH specifically hydroxylates an Asp or Asn residue in the epidermal growth factor-like (EGF) domains of several proteins, using iron as a cofactor. While all ASPH variants are expressed in skeletal muscle, only some are detected in heart, brain, pancreas, placenta, lung, liver, and kidney tissues. Recombinant human ASPH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
| Further Information: | Sequence: MGSSHHHHHH SSGLVPRGSH MFDLVDYEEV LGKLGIYDAD GDGDFDVDDA KVLLGLKERS TSEPAVPPEE AEPHTEPEEQ VPVEAEPQNI EDEAKEQIQS LLHEMVHAEH ETEHSYHVEE TVSQDCNQDM EEMMSEQENP DSSEPVVEDE RLHHDTDDVT YQVYEEQAVY EPLENEGIEI TEVTAPPEDN PVEDSQVIVE EVSIFPVEEQ QEVPPDT |
| Synonym: | Aspartyl/asparaginyl beta-hydroxylase, AAH, BAH, CASQ2BP1, HAAH, JCTN, junctin, Aspartyl/asparaginyl beta-hydroxylase ASP beta hydroxylase, Aspartyl/asparaginyl beta hydroxylase, Peptide aspartate beta dioxygenase, |
| Ascension Number: | Q12797 |
| NCBI EntrezGene: | NP_001158227 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
