Applications: | SDS-PAGE |
Species Reactivity: | E.coli |
MW: | 18.1 kDa (161aa), confirmed by MALDI-TOF. |
Purity: | > 95% by SDS - PAGE |
Description: | DsbE, also called CcmG, is a reducing Dsb protein implicated in electron transfer for cytochrome c maturation in the periplasm of Escherichia coli. DsbE is one of 12 proteins required for their assembly in the periplasm. Its postulated function is to reduce disulphide bonds formed between correctly paired cysteine residues in the cytochrome c apoproteins prior to haem attachment by CcmF and CcmH. Recombinant DsbE protein was expressed in E. coli and purified by using conventional chromatography. |
Further Information: | Sequence: MRNAEGDDPT NLESALIGKP VPKFRLESLD NPGQFYQADV LTQGKPVLLN VWATWCPTCR AEHQYLNQLS AQGIRVVGMN YKDDRQKAIS WLKELGNPYA LSLFDGDGML GLDLGVYGAP ETFLIDGNGI IRYRHAGDLN PRVWEEEIKP LWEKYSKEAA Q |
Synonym: | Thiol:disulfide interchange protein dsbE, CcmG, yejQ, Thiol:disulfide interchange protein dsbE Cytochrome c biogenesis protein ccmG..ECK2187, Periplasmic thioredoxin of cytochrome c type biogenesis, JW2183. |
Ascension Number: | P0AA86 |
NCBI EntrezGene: | NP_416699 |
Shipping Conditions: | Ship on cold packs |
Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Usage: | Research use only |