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Cat Number:ATGA0483-100ul
Size:100uL
Concentration:1 mg/mL
Price:£205.00
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Shipping:£12.00

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Calnexin [AT18B9], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, FACS, ICC/IF
Species Reactivity:Human
Isotype:Mouse IgG1 kappa
Immunogen:Recombinant human Calnexin (21-481aa) purified from E. coli.
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:Calnexin, also known as IP90, p88 and p90, is a member of the calnexin family of molecular chaperones. This protein is a calcium-binding, endoplasmic reticulum (ER) -associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.
Further Information:The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:CNX, IP90, P90, CANX, FLJ26570, Histocompatibility complex class I antigen binding protein p88,
Ascension Number:P27824
NCBI EntrezGene:NP_001019820
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Calnexin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: MCF7 cell lysate Lane 3.: A431 cell lysate Lane 4.: A549 cell lysateLane 5.: 293T cell lysateLane 6.: HepG2 cell lysateLane 7.: Jurkat cell lysate

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Calnexin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: MCF7 cell lysate Lane 3.: A431 cell lysate Lane 4.: A549 cell lysateLane 5.: 293T cell lysateLane 6.: HepG2 cell lysateLane 7.: Jurkat cell lysate

ICC/IF analysis of Calnexin in HeLa cells. The cell was stained with ATGA0483 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

ICC/IF analysis of Calnexin in HeLa cells. The cell was stained with ATGA0483 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).