| Applications: | ELISA, WB, FACS, ICC/IF |
| Species Reactivity: | Human |
| Isotype: | Mouse IgG2a kappa |
| Immunogen: | Recombinant human AKT1 (1-480aa) purified from insect cell. |
| Purification: | By protein-A affinity chromatography |
| Conjugation: | Unconjugated |
| Description: | AKT1, also known as RAC-alpha serine/threonine-protein kinase, is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. |
| Further Information: | The antibody has been tested by Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. |
| Synonym: | RAC-alpha serine/threonine-protein kinase, AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA |
| Ascension Number: | P31749 |
| NCBI EntrezGene: | NP_001014432 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
