Ordering Details
Cat Number:	ATGA0466-50ul
Size:	50uL
Concentration:	1 mg/mL
Price:	£123.00
Quantity:
Shipping:	£12.00

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MSDS

PGC1 alpha [AT25C8], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, FACS, ICC/IF
Species Reactivity:	Human
Isotype:	Mouse IgG2a kappa
Immunogen:	Recombinant human PPARGC1A (300-540aa) purified from E. coli
Purification:	By protein-A affinity chromatography
Conjugation:	Unconjugated
Description:	PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. This protein can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Also, induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner and involved in the integration of the circadian rhythms and energy metabolism. They required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.
Further Information:	The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Ascension Number:	Q9UBK2
NCBI EntrezGene:	NP_037393
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPARGC1A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: MCF7 cell lysate Lane 3.: HepG2 cell lysate Lane 4.: 293T cell lysateLane 5.: Mouse brain tissue lysate

ICC/IF analysis of PPARGC1A in HeLa cells. The cell was stained with ATGA0466 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).