The HepG2 cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACSF2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
ICC/IF analysis of ACSF2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACSF2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)