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Cat Number:ATGA0462-50ul
Size:50uL
Concentration:1 mg/mL
Price:£123.00
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Shipping:£12.00

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Aminopeptidase P1/XPNPEP1 [AT9C7], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, ICC/IF
Species Reactivity:Human
Isotype:Mouse IgG2b kappa
Immunogen:Recombinant human XPNPEP1 (1-623aa) purified from E. coli
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:XPNPEP1 also known as Xaa-Pro aminopeptidase 1 isoform 1, is encodes the cytosolic form of a metalloaminopeptidase that catalyzes the cleavage of the N-terminal amino acid adjacent to a proline residue. This product may play a role in degradation and maturation of tachykinins, neuropeptides, and peptide hormones.
Further Information:The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:Xaa-Pro aminopeptidase 1 isoform 1, APP1, SAMP, XPNPEP, XPNPEPL, XPNPEPL1
Ascension Number:Q9NQW7
NCBI EntrezGene:NP_065116
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human XPNPEP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : MCF7 cell lysate

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human XPNPEP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : MCF7 cell lysate

CC/IF analysis of XPNPEP1 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

CC/IF analysis of XPNPEP1 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).