The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human XPNPEP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : MCF7 cell lysate
CC/IF analysis of XPNPEP1 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).