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Cat Number:ATGA0459-100ul
Size:100uL
Concentration:1 mg/mL
Price:£205.00
Quantity:
Shipping:£12.00

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ACADS [AT8B10], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, ICC/IF
Species Reactivity:Human
Isotype:Mouse IgG2a kappa
Immunogen:Recombinant human ACADS (25-412aa) purified from E. coli
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:ACADS (Acyl-Coenzyme A dehydrogenase), also known as SCAD or ACAD3, is a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations of ACADS have been associated with fatty acid oxidation defects and metabolic diseases such as short-chain acyl-CoA dehydrogenase deficiency (SCAD deficiency).
Further Information:The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:Acyl-Coenzyme A dehydrogenase, ACAD3, SCAD, Acyl-Coenzyme A dehydrogenase Acyl Coenzyme A dehydrogenase, C2 to C3 short chain, Acyl-CoA dehydrogenase, C2 to C3 short chain, Acyl-CoA dehydrogenase, short chain, Acyl-Coenzyme A dehydrogenase, short chain, AI196007, Bcd-1, Bcd1, Butyryl CoA dehydrogenase, EC 1.3.99.2, Short chain acyl CoA dehydrogenase, unsaturated acyl CoA reductase, Short-chain specific acyl-CoA dehydrogenase, mitochondrial.
Ascension Number:P16219
NCBI EntrezGene:AAH25963
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACADS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : HepG2 cell lysateLane 2 : LnCap cell lysate

The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACADS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : HepG2 cell lysateLane 2 : LnCap cell lysate

ICC/IF analysis of ACADS in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACADS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).ICC/IF analysis of ACADS in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human  ACADS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of ACADS in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACADS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).ICC/IF analysis of ACADS in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACADS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).