Ordering Details
Cat Number:	ATGA0453-100ul
Size:	100uL
Concentration:	1 mg/mL
Price:	£205.00
Quantity:
Shipping:	£12.00

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MSDS

Vimentin [AT5H8], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, ICC/IF
Species Reactivity:	Human
Isotype:	Mouse IgG1 kappa
Immunogen:	Recombinant human VIM (1-466aa) purified from E. coli
Purification:	By protein-A affinity chromatography
Conjugation:	Unconjugated
Description:	Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all metazoan cells as well as bacteria. IF, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastaticprogression.
Further Information:	The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:	Vimentin, epididymis luminal protein 113, HEL113, CTRCT30
Ascension Number:	P08670
NCBI EntrezGene:	NP_003371
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VIM antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : Jurkat cell lysateLane 2 : K562 cell lysateLane 3 : 293T cell lysateLane 4 : A549 cell lysateLane 5 : SK-OV-3 cell lysateLane 6 : HeLa cell lysate

ICC/IF analysis of VIM in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human VIM antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).