The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: U87-MG cell lysate Lane 3.: K562 cell lysate Lane 4.: 293T cell lysate Lane 5.: HepG2 cell lysate Lane 6.: A549 cell lysate Lane 7.: MCF7cell lysate Lane 8.: SK-OV-3 cell lysate Lane 9.: PC3 cell lysate
ICC/IF analysis of AK1 in HeLa cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).ICC/IF analysis of AK1 in Hep3B cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).ICC/IF analysis of AK1 in A549 cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
