Ordering Details
Cat Number:	ATGA0438-100ul
Size:	100uL
Concentration:	1 mg/mL
Price:	£205.00
Quantity:
Shipping:	£12.00

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MSDS

BAG2 [AT29E9], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, ICC/IF
Species Reactivity:	Human
Isotype:	Mouse IgG2a kappa
Immunogen:	Recombinant human BAG2 (1-211aa) purified from E. coli
Purification:	By protein-A affinity chromatography
Conjugation:	Unconjugated
Description:	BCL2-associated athanogene 2, also known as BAG2, is a member of the Bag family of proteins. BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain. BAG2 is a major component of the HSC 70/CHIP chaperone-dependent ubiquitin ligase complex and acts to disrupt CHIP-mediated ubiquitylation.
Further Information:	The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:	BCL2-associated athanogene 2, BAG-2
Ascension Number:	O95816
NCBI EntrezGene:	NP_004273
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BAG2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Jurkat cell lysateLane 2.: A549 cell lysate Lane 3.: K562 cell lysate Lane 4.: HepG2 cell lysate Lane 5.: HeLa cell lysate

ICC/IF analysis of BAG2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BAG2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).