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Cat Number:ATGA0436-100ul
Size:100uL
Concentration:1 mg/mL
Price:£205.00
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Shipping:£12.00

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FKBP14 [AT18E2], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, ICC/IF
Species Reactivity:Human
Isotype:Mouse IgG1 kappa
Immunogen:Recombinant human FKBP14 (20-211aa) purified from E. coli
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:FKBP14, also known as 22 kDa FK506-binding protein, is an enzyme that accelerates the folding of proteins during protein synthesis. This protein contains two EF-hand domains and one PPIase FKBP-type domain. Truncation of the amino-terminus of FKBP14 greatly reduces peptidyl prolyl cis-trans isomerase activity, therefore suggesting that the PPIase FKBP-type domain must be located at the N-terminus.
Further Information:The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:Peptidyl-prolyl cis-trans isomerase FKBP14, FKBP22, Peptidyl-prolyl cis-trans isomerase FKBP14, 22 kDa FK506 binding protein, FK506 binding protein 14 (22 kDa), FK506 binding protein 14, FKBP 22, FKBP22, Peptidyl prolyl cis trans isomerase, PPIase, Rotamase
Ascension Number:Q9NWM8
NCBI EntrezGene:NP_060416
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP14 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : HeLa cell lysate The cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP14 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : 293T cell lysate Lane 2. : FKBP14 Transfected 293T cell lysate

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP14 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : HeLa cell lysate The cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP14 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : 293T cell lysate Lane 2. : FKBP14 Transfected 293T cell lysate

ICC/IF analysis of FKBP14 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human   FKBP14 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of FKBP14 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FKBP14 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).