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Cat Number:ATGA0429-100ul
Size:100uL
Concentration:1 mg/mL
Price:£205.00
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Shipping:£12.00

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ACAA1 [AT9E5], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, ICC/IF
Species Reactivity:Human
Isotype:Mouse IgG2b kappa
Immunogen:Recombinant human ACAA1 (27-424aa) purified from E. coli
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:ACAA1 is a member of the thiolase family of enzymes and is involved in lipid metabolism. This protein is localized to the peroxisome and catalyzes the conversion of acyl-CoA and acetyl-CoA to 3-oxoacyl-CoA in the fatty acid oxidation pathway. ACAA1 shows high enzymatic activity in liver, kidney, intestine and white adipose tissue in rats. Deficiency of this enzyme leads to pseudo-Zellweger syndrome.
Further Information:The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:3-ketoacyl-CoA thiolase peroxisomal, ACAA, PTHIO, THIO, 3-ketoacyl-CoA thiolase, peroxisomal
Ascension Number:P09110
NCBI EntrezGene:NP_001598
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAA1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : HepG2 cell lysateLane 2 : LnCap cell lysate

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAA1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : HepG2 cell lysateLane 2 : LnCap cell lysate

ICC/IF analysis of ACAA1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human   ACAA1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of ACAA1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACAA1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).