Ordering Details
Cat Number:	ATGA0426-50ul
Size:	50uL
Concentration:	1 mg/mL
Price:	£123.00
Quantity:
Shipping:	£12.00

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Creatine Kinase BB [AT8E4], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, FACS, ICC/IF
Species Reactivity:	Human
Isotype:	Mouse IgG2a kappa
Immunogen:	Recombinant human CKB (1-381aa) purified from E. coli
Purification:	By protein-A affinity chromatography
Conjugation:	Unconjugated
Description:	CKB (Creatine kinase B-type) is a cytoplasmic enzyme involved in energy homeostasis. The protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. Creatine kinases provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems including muscle, tumor and cancer cells.
Further Information:	The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:	Creatine kinase B-type, B-CK, CKBB, Creatine kinase B-type, BCK
Ascension Number:	P12277
NCBI EntrezGene:	NP_001814
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CKB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: 293T cell lysate Lane 2.: K562 cell lysate Lane 2.: LnCap cell lysate

ICC/IF analysis of CKB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CKB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).