Ordering Details
Cat Number:	ATGA0424-100ul
Size:	100uL
Concentration:	1 mg/mL
Price:	£205.00
Quantity:
Shipping:	£12.00

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MSDS

Enolase 1, 2 [AT1G7], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, FACS, ICC/IF
Species Reactivity:	Human
Isotype:	Mouse IgG2a kappa
Immunogen:	Recombinant human Alpha-enolase (1-434aa) purified from E. coli
Purification:	By protein-A affinity chromatography
Conjugation:	Unconjugated
Description:	Alpha-enolase, also known as Enolase 1, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of alpha-enolase is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoantigen associated with Hashimoto's encephalopathy and severe asthma.
Further Information:	The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:	Non-Neuronal Enolase (NNE), Enolase-1, ENO1, MBP-1, MYC promoter-binding protein 1, PPH, tau-crystallin, 2 phospho D glycerate hydro lyase, Alpha enolase, C myc promoter binding protein, EC 4.2.1.11, ENO1L1, Enolase 1, Enolase 1 (alpha), Enolase 1 (alpha) like 1, MBP 1, MBP1, MBPB1, MPB 1, MPB1, PPH, MYC promoter binding protein 1, NNE, Non neural enolase, Phosphopyruvate hydratase, Plasminogen binding protein,
Ascension Number:	P06733
NCBI EntrezGene:	NP_001419
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ENO1, 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: ENO1 (Alpha-enolase) recombinant proteinLane 2.: ENO2 (Gamma-enolase) recombinant proteinLane 3.: ENO3 (Beta-enolase) recombinant protein The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ENO1, 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: PC3 cell lysateLane 2.: MCF7 cell lysateLane 3.: 293T cell lysateLane 4.: HeLa cell lysate

ICC/IF analysis of ENO1, 2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ENO1, 2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).