Ordering Details
Cat Number:	ATGA0416-100ul
Size:	100uL
Concentration:	1 mg/mL
Price:	£205.00
Quantity:
Shipping:	£12.00

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MSDS

ASS1 [AT1G11], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, ICC/IF
Species Reactivity:	Human
Isotype:	Mouse IgG2a kappa
Immunogen:	Recombinant human ASS1 (1-412aa) purified from E. coli
Purification:	By protein-A affinity chromatography
Conjugation:	Unconjugated
Description:	ASS1 (argininosuccinate synthase or citrulline-aspartate ligase) belongs to the argininosuccinate synthase family. ASS1 is a urea cycle enzyme that is essential in the conversion of nitrogen from ammonia and aspartate to urea. A deficiency of ASS1 causes citrullinemia (CTLN1), an autosomal recessive disease which is characterized by severe vomiting spells and mental retardation. Cells deficient in ASS1 must absorb the arginine they need for growth from circulating blood.
Further Information:	The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:	Argininosuccinate synthase, ASS, CTLN1, Argininosuccinate synthase
Ascension Number:	P00966
NCBI EntrezGene:	NP_000041
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The MCF-7 cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ASS1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ICC/IF analysis of ASS1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ASS1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).